Application

This Anti-c-Ret Antibody, clone 6F3.1 is validated for use in Western Blotting for the detection of c-Ret.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Proto-oncogene tyrosine-protein kinase receptor Ret (EC 2.7.10.1; UniProt P07949; also known as Cadherin family member 12, Cadherin-related family member 16, Hydroxyaryl-protein kinase, Proto-oncogene c-Ret, Rearranged during transfection, Receptor tyrosine kinase, Ret proto-oncogene, RET receptor tyrosine kinase, RET transforming sequence) is encoded by the RET (also known as CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, RET51) gene (Gene ID 5979) in human. Ret is a receptor tyrosine kinase that mediates the activation of several signaling pathways including the PLC gamma, Ras, JNK and inositol phosphate pathways. Ret mutations have been shown to be causative in several diseases, including Hirschsprung′s disease (HD), papillary thyroid carcinoma, multiple endocrine neoplasia (MEN) 2A, MEN 2B, and familial medullary thyroid carcinoma. Ret is initially produced with a signal peptide sequence (a.a. 1-28), the removal of which yields the mature Ret (a.a. 20-1114). Ret can be posttranslationally cleaved into the extracellular cell-membrane-anchored RET cadherin 120 kDa fragment (a.a. 29-707) and the soluble RET kinase fragment (a.a. 708-1017).

Immunogen

Epitope: Extracellular domain

GST-tagged recombinant protein corresponding to the extracellular domain of human c-Ret.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected c-Ret in 10 µg of PC12 cell lysate.

Specificity

Expected to react with both the RET51 and the RET9 isoforms, as well as the extracellular cell-membrane anchored RET cadherin 120 kDa fragment (a.a. 29-707).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150 kDa observed. 124.3 kDa (RET51) and 119.8 kDa (RET9) calculated. Target band(s) may appear larger than the calculated molecular size(s) due to posttranslational glycosylation and/or phosphorylation. Uncharacterized band(s) may appear in some lysates.